The method is primarily used in collaborative research projects, and other analytical services are agreed on a case by case basis. Written requests and contacts:
Environmental pollutant analysis

Cotinine is a nicotine metabolite, which is used as an indicator of tobacco smoke exposure. Cotinine can be detected in the blood and urine of both active and passive smokers. Cotinine is often analyzed together with the method Pollutants from small serum volume:
Pollutants from small serum volume (micro POP)


  • The method is accredited for urine and serum

Number of samples

  • Minimum 6 samples


  • Urine
  • Serum

Method description

  • Analytes are extracted with an organic solvent. The quantification is performed with a liquid chromatograph coupled to a triple quadrupole mass spectrometer (LC-MS/MS).

Limit of quantification

  • 0.25 ng/ml

Sampling instructions

  • Sample container: glass or polypropylene (PP)
  • Minimum sample amount: serum 0.1 ml, urine 1.0 ml
  • Maximum storage time: +7°C for 3 days, -18°C for at least 20 years, -70°C or in liquid nitrogen indefinitely
  • See also the general sampling instructions that apply to all analyses:
    Environmental pollutant analysis


  • Frozen in cold package


  • Cotinine

FINAS and ILAC MRA accreditation symbols.

THL's scope of accreditation

The unit is a FINAS accredited testing laboratory T077. It has been accredited according to the standard SFS-EN ISO/IEC 17025:2005 and its scope of accreditation is available on the website of FINAS (Finnish Accreditation Service).

Work order

The work order must be included in the shipment of samples along with an itemized sample list, if needed.

Work order for environmental pollutant analyses (PDF 132 KB)

On our website