PBDEs

The method is primarily used in collaborative research projects, and other analytical services are agreed on a case by case basis. Written requests and contacts:
Environmental pollutant analysis

See also Pollutants from small serum volume: 
Pollutants from small serum volume (micro POP)

Accreditation

• The method is accredited with a flexible scope for all matrices and analytes listed below

Number of samples

• Also for single samples

Matrices

• Serum, breast milk, placenta and other human samples
• Fish and other animal samples
• Food and feed
• Soil, sediment and other environmental samples suitable for routine analysis
• Water
• Other samples containing fat or oil

Method description

• After matrix-specific pretreatment, analytes are extracted and the extract is cleaned with a silica and aluminium oxide column. The analytes are separated with an active carbon column. The quantification is performed with a gas chromatograph coupled to a triple quadrupole mass spectrometer (GC-MS/MS).

Limit of quantification

• Depends on the sample amount and matrix
• For example PBDEs in tissues: 10–100 pg/g fat

Sampling instructions

• Sample container: glass, polyethene (PE) or aluminium foil
• Avoid contact with plastics
• Minimum sample amount: liquids 1000 ml, serum and breast milk 100 ml, soil and sediment 50 g, food and feed 1000 g (EU N:o 252/2012), fat 10 g
• Maximum storage time (water, soil and sediment): +7°C for 7 days, -18°C for at least 20 years, -70°C or in liquid nitrogen indefinitely
• Maximum storage time (other samples): +7°C for 3 days, -18°C for at least 20 years, -70°C or in liquid nitrogen indefinitely
• See also the general sampling instructions that apply to all analyses:
  Environmental pollutant analysis

Dispatch

• Frozen in cold package

Analytes

• PBDE 28
• PBDE 47
• PBDE 49
• PBDE 66
• PBDE 77
• PBDE 99
• PBDE 100
• PBDE 138
• PBDE 153
• PBDE 154
• PBDE 183
• PBDE 209